Avocado Sunblotch Viroid Detection ASBVd is found in avocado growing regions worldwide. Trees infected with ASBVd can result in a loss of yield and / or production of unmarketable fruit. However, symptoms are not always present making pro-active testing a critical disease control measure. Available kit types for Avocado Sunblotch Viroid Nucleic Acid Hybridization Assay Agdia-Emea’s ASBVd Nucleic Acid Hybridization Assay is a convenient and economical method for testing hundreds of trees at once. Its high sensitivity allows combining up to 8 leaves from each of 4 trees, or a total of 32 leaves, for analysis. The assay’s limit of detection is approximately 12.5 picograms of in vitro expressed ASBVd RNA.