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Agdia Now Accepting Hemp Samples for Pathogen Testing

Following decades of service to food crop and ornamental growers, Agdia’s in-house diagnostic laboratory is now offering its expansive disease testing program to the hemp market.

Deborah (Debi) Groth-Helms is the Director of Agdia’s Testing Services department. “We are excited to finally offer our comprehensive virus testing program to hemp growers and diagnosticians. For years we have worked with stock and seed producers in screening propagative tissues for the presence of key pathogens. Now we can help bolster the US hemp industry in their efforts to produce the healthiest plants possible.”

Agdia Testing Services offers multiple screening options and works with clients in customizing screens to best suit their needs. The basic Hemp Screen includes a panel of a dozen different tests for viruses, viroids, and other microorganisms.

According to Debi, “Information is being rapidly released on what causes disease in Cannabis. Having a big catalog of services already in place allows us to quickly respond to new reports. Experience working with closely related plant species helps us know what to monitor before an outbreak occurs.”

Included in the Hemp Screen are tests for Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), Hop latent viroid (HLVd), and many others. The Curto-Becurtovirus Group test listed in the screen detects all of the various virus species associated with Beet curly top disease. Additional tests are also available for less common or emerging pathogens. These highly sensitive molecular assays can be used to detect diseases such as witches’ broom (Phytoplasma Group PCR) and Lettuce chlorosis virus (Closteroviridae Group PCR).

Currently the laboratory is only accepting hemp tissues from US submitters that hold an active hemp license through the USDA, their state government, or tribal entity.  Information on hemp licensing and the 2018 Farm Bill can be found on the USDA’s website. The laboratory recommends shipping plant material with the United States Postal Service (USPS) along with a completed sample submission form.

Information on how to send samples can be found on the Agdia website. Questions regarding samples, customized screens, requests for quotes, and all other inquiries can be sent to info@agdia-emea.com

Agdia Commercializes ToBRFV ImmunoStrip® to Complete Diagnostic Suite

Agdia, Inc. (Elkhart, IN) has added an to their Tomato brown rugose fruit virus (ToBRFV) diagnostic suite. Their ImmunoStrip® for ToBRFV is the third diagnostic assay launched by Agdia in the first half of 2021 for detection of this pathogen. Their high-specificity ELISA assay for ToBRFV was released on January 7, followed by a rapid molecular AmplifyRP® XRT for ToBRFV on January 12.

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Tomato brown rugose fruit virus is a resistance-breaking Tobamovirus that causes severe economic losses in solanaceous crops, including Solanum lycopersicum (tomato) and Capsicum spp. (pepper). It causes symptoms typical of Tobamoviruses that include mosaic and chlorosis on the leaves and discoloration and deformation of the fruit.  These symptoms decrease yield and render fruit unmarketable.

Tomato and pepper seeds, transplants and fruits from certain countries are subject to a USDA-APHIS Federal Import Order in the United States. Tomato brown rugose fruit virus has also been classified as a quarantine pathogen by EPPO (European and Mediterranean Plant Protection Organization).

Agdia’s ImmunoStrip® for ToBRFV has been validated for use with tomato, pepper and petunia samples and has been tested against ToBRFV isolates from around the globe.

Cross-reactivity was observed with a Tomato mottle mosaic virus (ToMMV) sample. Very mild cross-reactivity was observed with Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) samples. No other cross-reactions were observed when testing other Tobamoviruses, including Bell pepper mosaic virus (BPeMV), Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), Pepper mild mottle virus (PMMoV), Tobacco mild green mosaic virus (TMGMV), and Zucchini Green Mottle Mosaic Virus (ZGMMV).

Agdia’s ImmunoStrip® platform provides end-users with a high level of utility. ImmunoStrips® are fit for use in the lab or in the field by those having no previous diagnostic experience, and results are visualized within 30 minutes.  Increasing levels of market demand persists for field-deployable plant pathogen detection products and Agdia maintains they will continue to expand this area of their catalog.

Agdia Adds to Diagnostic Suite for ToBRFV with Release of a Rapid, RNA-based Isothermal Molecular Kit

Agdia, Inc. (Elkhart, IN) has added another test kit to their Tomato brown rugose fruit virus (ToBRFV) diagnostic suite. AmplifyRP® XRT for ToBRFV is the second of three ToBRFV diagnostic assays to be launched by Agdia in the first half of 2021. Their high-specificity ELISA assay for ToBRFV was released on January 7. An ImmunoStrip® lateral flow device for ToBRFV is expected to be released in the coming months (Q1 or Q2 of 2021.)

Agdia’s AmplifyRP® XRT for ToBRFV has been validated for use with tomato and pepper seeds and leaf. As a rapid, field-deployable molecular method requiring far less training than traditional PCR methods, this assay provides users with greater flexibility to deploy detection capabilities where they need it, when they need it. Use cases for this assay include:

  • Laboratory-based molecular diagnosis with crude or purified extracts with faster time-to-result than traditional PCR or qPCR methods. This assay can be used with Agdia’s AmpliFire® isothermal fluorometer or with most commonly-used real-time PCR machines.
  • In-field monitoring at remote production sites as a stand-alone assay.
  • Directly test ELISA extracts for molecular confirmation of serological screening results, allowing you to act immediately to address production issues.

Agdia’s AmplifyRP® XRT for ToBRFV is highly specific to ToBRFV and has been experimentally proven to detect isolates from around the world. No cross-reactivity was observed with high titer samples from other Tobamoviruses, including Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), Pepper mild mottle virus (PMMoV), Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV), Tobacco mild green mosaic virus (TMGMV), Zucchini Green Mottle Mosaic Virus (ZGMMV) and more.

Tomato brown rugose fruit virus is a resistance-breaking Tobamovirus that causes severe economic losses in solanaceous crops, including Solanum lycopersicum (tomato) and Capsicum spp. (pepper). It causes symptoms typical of Tobamoviruses that include mosaic and chlorosis on the leaves and discoloration and deformation of the fruit.  These symptoms decrease yield and render fruit unmarketable.

Tomato and pepper seeds, transplants and fruits from certain countries are subject to a USDA-APHIS Federal Import Order in the United States. Tomato brown rugose fruit virus has also been classified as a quarantine pathogen by EPPO (European and Mediterranean Plant Protection Organization).

Detect Potyvirus in 30min with Flashkit®

We are pleased to announce that we have successfully developed and commercialized the industries first rapid diagnostic, the POTYVIRUS Flashkit®, for detection of Potyviruses.
The Potyvirus genus consists of at least 118 viruses that can infect hundreds of plant species. Potyviruses are aphid transmitted, although other means of transmission are possible, which contributes to the rapid spread of the pathogen in host crops. Symptoms will vary among infected plants depending on the host and the Potyvirus that has infected it. Affected crops may perform poorly in the field and / or produce unmarketable fruit. Some Potyviruses, like Plum pox virus, also have regulatory impacts if detected.

Flashkit PotyvirusAgdia-Emea’s POTY Flashkit® provides growers a new tool for detection and surveillance of Potyviruses. The test has been validated to detect more than 40 viruses within the Potyvirus genus and can be used to test more than 50 crops. That list will continue to expand as more validation data is produced. Flashkit® tests are grower-friendly and do not require special laboratory skills to use. The Flashkit® test is comparable in sensitivity and reliability to other antibody-based laboratory methods such as ELISA.
To perform the test a user would tear off a piece of suspicious plant material and insert it into an extraction bag included with a test kit. Once the plant tissue has been extracted by rubbing it with a pen or marker, the Flashkit® is inserted directly into the bag which initiates the test. As the test runs, one or two lines will appear indicating a negative or positive result, respectively. We recommend allowing the test to run 30 minutes, but positive results may be visible in 5 minutes or less.
The POTY Flashkit® is offered in kits of 5 and 25 tests. Flashkit® includes everything necessary to perform a test except scissors and is warranted for 1 year.

ImmunoStrips is a registered trademark of Agdia Inc.. ImmunoStrips are trademarked and marketed in EMEA under the name Flashkit.

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